|
KMID : 0383919740110000081
|
|
Report of National Institute of Health
1974 Volume.11 No. 0 p.81 ~ p.88
|
|
|
Antibody Survey against Poliomyelitis Virus of Korean, 1974
|
|
金慶浩/Kim, K.H.
白承福/張起峰/申榮午/白遠鴻/Paik, S.B./Chang, K.B./Shin, Y.O./Baeg, W.H.
|
|
|
Abstract
|
|
|
In 1974 poliomyelitis virus autibody svrvey was carried on to determine the levels of different age groups in Seoul and the results were compared with the data which had been investigated ini 1964.
Technics was used neutralization antibody test in monkey kidney cell(Vero) culture by means of microtitration method. For neutralization test, Mahoney(Type Ⅰ), MEF-1 (Type Ⅱ), and Saukett(Type Ⅲ) strains of Poliomyelitis viruses were used. Each serum were diluted 1 : 4 in Hank´s balanced salt solution.
Two-tenths ml of diluted serurn from each human volunteer was mixed with 0.2mi of the virus suspension which contained 100 TCID_(50) per 0.1ml cultured in Vero cells for 5-7 days. After keeping the serum-virus mixture at 37℃ for 1 hour and overnight at 4℃, 0.05ml of the mixture was inoculated into each of 0.05ml containing Vero cells.
The results were read microscopically after 5-7 days incubation at 37℃. in each serological test, virus titeration with the 60 units of reference serum of known antibody was carried on. Two hundred and fifteen single blood specimens were collected from persons who had no paast history of clinical poliomyelitis in September to November, 1974.
The results obtained were as follows;
1. It was indicated that the maternal antibody was detected until 6 month after birth and shortly after the loss of the maternal immunity. This results was almost similar with the results of ten years age.
2. By the time the infants 6-12 month old, 57.0 per cent of them had antibodies against three types of poliomyelitis uirus and then the antibodies positive rate were increased with age increasing. Ten years old children group had triple antibodies over 90.0 per cent. In compared with the results of ten years age, it is noteworthy that the triple antibodies positive rate in 1974 were quite higher than in 1964 (23.0%). It is suggested that polio sabin type vaccine was used nation widely since 1963 to the infants.
3. Among the maternal immunity, type Ⅰ viurs antibody positive rate was more higher than the others and it was showed that the infants who had 100.0 per cent decreased as to 68.8 per cent by 12 months after birth and then increase as to 80.0 per cent by 2 years old.
4. It was showed that type Ⅱ virus maternal antibody was the most lower rate (81.3 per cent). It was indicated that only 71.4 per cent positive rate was detected in the 3 years age group.
5. Type Ⅲ virus maternal antibody positive rate was 94.3 per cent in the 7-12 month age group and positive rate was increased as to 80.0 per cent in the 2 years age group and then antibody positive rate was increased by following the increasing ages.
6. In conclusion, it is recommened that all the children under 4 years old should inoculated with the polio sabin type vaccine at the same time, and then newborn child should inoculated with the vaccine every year after at least 7 months after birth when the maternal antibody disappeared.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|